Calibration support in the Atmel-ICE, AVRISP mkII, JTAGICE3, and JTAGICE mkII tools is only supported in the command-line tool atprogram. In the Batch file sub-directory, batch files are provided that use atprogram to program the calibration code into the target device, perform the calibration and then reprogram the device with the final firmware. The batch files perform calibration of the device through the Atmel-ICE, AVRISP mkII, JTAGICE3, and JTAGICE mkII.