The recommended pinout for the 6-pin TPI connector is shown in AVR TPI Pinout.
Connection to a 6-pin 100-mil TPI Header
Use the AVR 6-pin adapter board to connect to a standard 100-mil TPI header.
Connection to a 6-pin 50-mil TPI Header
Use the AVR 6-pin mini adapter board to connect to a standard 50-mil TPI header.