8.3.9.3.3 Transfer Speed/Bit Rate
The TWI speed is defined in FLEX_TWI_CWGR. The TWI bit rate can be based either on the peripheral clock if the BRSRCCLK bit value is 0 or on a programmable clock source provided by the GCLK if the BRSRCCLK bit value is 1.
If BRSRCCLK = 1, the bit rate is independent of the processor/peripheral clock and thus processor/peripheral clock frequency can be changed without affecting the TWI transfer rate.
The GCLK frequency must be at least three times lower than the peripheral clock frequency.