27.3.2.2 Bit Transfer
As illustrated by Figure 27-5, a bit transferred on the SDA line must be stable for the entire high period of the SCL line. Consequently, the SDA value can only be changed during the low period of the clock. This is ensured in hardware by the TWI module.
Combining bit transfers result in the formation of address and data packets. These packets consist of eight data bits (one byte) with the Most Significant bit transferred first, plus a single-bit not-Acknowledge (NACK) or Acknowledge (ACK) response. The addressed device signals ACK by pulling the SCL line low during the ninth clock cycle, and signals NACK by leaving the line SCL high.