34.5.1 I/O Lines

To use the SERCOM’s I/O lines, the I/O pins must be configured as direct using the System Configuration registers. (See System Configuration and Register Locking (CFG) from Related Links.) I2C does not operate through PPS. (See the DEVCFG1 configuration bits SCOMn_HSEN in Configuration Bits Fuses and also the CFGCON1 SCOMn_HSEN in CFGCON1(L) register.)
Note: SERCOM2 has only I2C functionality and, therefore, the direct configuration using System configuration register is not required.

When the SERCOM is used in I2C mode, the SERCOM controls the direction and value of the I/O pins. Both PORT control bits PINCFGn.PULLEN and PINCFGn.DRVSTR are still effective. In I2C mode, pull-up resistors are disabled. External pull-up resistors are required for proper function.