The STK500 does not have a USB connection, and Microchip Studio cannot automatically detect it. Therefore, add it to the list of available tools before using it by the Device Programming window.
To add an STK500, right click inside the Available Tools view, select Add target , and select STK500 as the tool, and to which COM port your STK500 will be connected.
Press the Apply button, and the STK500 will show up in the list of available tools.
Note: An added STK500 will be visible in the
Available Tools view event even without any STK500 connected to the specified COM
port.
If you want to remove STK500 from the list, you can right click on it and select Remove from the context menu.